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Monsanto Technology LLC
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BioSpec
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Image Search Results
Journal: Basic & clinical pharmacology & toxicology
Article Title: Effects of insulin-like growth factor-1 on rotenone-induced apoptosis in human lymphocyte cells.
doi: 10.1111/j.1742-7843.2009.00472.x
Figure Lengend Snippet: Fig. 4. Rotenone (ROT) induced simultaneous NF-jB, p53 and c-Jun transcription factor activation in lymphocytes. Peripheral blood lymphocytes cells were left untreated (A–C), exposed to 250 lM rotenone (D–F), 250 nM IGF-1 (G–I), and to 250 nM IGF-1 + 250 lM rotenone (J–L) for 24 hr. After the incubation period, cells were stained with anti-NF-jB-p65 (A,D,G,J), anti-p53 (B,E,H,K) and anti-c-Jun (C,F,I,L) antibodies according to the procedure described in Materials and Methods. Notice that NF-jB, p53 and c-Jun positive-nuclei (dark brown) reflect their nuclear translocation ⁄ activation. Magnification 400· (A–L).
Article Snippet: The supplier’s protocol (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA; goat ABC staining System: cat # sc-2023) was followed for the immunocytochemistry using
Techniques: Activation Assay, Incubation, Staining, Translocation Assay